MEMBRANE PROTEINS OF KNOWN STRUCTURE

This compilation lists the membrane proteins of known structure, including crystallization conditions and key references for the structure determinations. It is continuously updated. The membrane proteins listed are divided into polytopic membrane proteins of the helical type from inner membranes of bacteria and mitochondria, and from eukaryotic membranes (A), the ß-stranded membrane proteins from the outer membrane of gram negative bacteria and related membrane proteins (B) and monotopic membrane proteins (C); these latter ones are proteins that are only inserted into the membrane, but do not cross it, (D) lists membrane proteins of uncertain classification. Within A, B and C the membrane proteins are listed in chronological order of the first structure determination. Statistics (S) of crystallization conditions can be found after D.
Structure determinations which led to C-alpha-coordinates only (1QO1, 1FE1, 1JSQ, 1MXM, 1PYH, 1QZV)  are not included.
 
 
CURRENT COUNT
OF  MEMBRANE PROTEIN  STRUCTURES:
 A:  58 different helical-type membrane proteins, among them 26 unrelated ones
B:  29 different ß-stranded membrane proteins, 1 two-stranded, 2 four-stranded, 1 seven-stranded, 4 eight-stranded, 2 ten-stranded, 3 twelve-stranded, 1 fourteen-stranded, 7 sixteen-stranded, 3 eighteen-stranded, 5 twenty two-stranded ß-barrel proteins (per monomer each)

C:  5, among them three unrelated ones

D: 1


Last update: March 30, 2006

Latest additions:
Methane monooxygenase from Methylococcus capsulatus (21-04-2005)
Outer membrane pyoverdine receptor FpvA from Pseudomonas aerugionsa (21-04-2005)
Outer membrane acyltransferase PagP from Escherichia coli (21-04-2005)
Outer membrane translocator domain of the autotransporter NalP from Neisseria meningitidis (21-04-2005)
TolC-like discharge outer membrane protein OprM from Pseudomonas aerugina (17-01-2005)
Bovine rhodopsin structure from MRC, Cambridge (13-01-2005)
Second structure of the ammonia transport protein AmtB from Escherichia coli (13-01-2005)
Sensory rhodopsin from Anabaena (13-01-2005)
Ca-ATPase from rabbit muscle complexed with Magnesiumfluoride (13-01-2005)
Outer membrane protein TolC from Escherichia coli complexed with hexammine cobalt (13-01-2005)
Outer membrane nucleoside transporter Tsx from Escherichia coli (25-11-2004)
A glutamate transporter homologue from Pyrococcus horikoshii (25-10-2004)
Ammonia transport protein AmtB from Escherichia coli (25-10-2004)
Aquaporin 0 from bovine eye lenses (25-10-2004)
A greatly improved structure of bovine rhodopsin (25-10-2004)
Cytochrome bc1 complex from Rhodobacter capsulatus (25-10-2004)
Ca-ATPase from rabbit muscle complexed with nucleotides (15-06-2004)
Long-chain fatty acid transporter FadL from Escherichia coli (14-06-2004)
Outer membrane channel MspA from Mycobacterium smegmatis (23-04-2004)
Photosystem II from Thermosynechococcus elongatus (24-03-2004)
Light-harvesting complex II from spinach chloroplasts (24-03-2004)
Protein-conducting SecY complex from Methanococcus jannaschii (12-03-2004)
Aquaporin Z from Escherichia coli (12-03-2004)
Chloride channel from Escherichia coli complexed with an antibody Fab-fragment (12-03-2004)
Improved coordinates from bovine heart cytochrome c oxidase (21-01-2004)
 
    

MEMBRANE PROTEINS AND THEIR CRYSTALLIZATION CONDITIONS:
 
Membrane Protein: Crystallization Conditions
(detergent/additive/precipitating agent)		 Key References 
(and pdb identifier, if available) 



A. Polytopic membrane proteins from inner membranes of bacteria and mitochondria, and from eukaryotic membranes






Photosynthetic reaction centres and photosystems:
Photosynthetic reaction centre

from Rhodopseudomonas viridis N,N-dimethyldodecylamine-N-oxide/  Deisenhofer et al., 1985

heptane-1,2,3-triol/ammonium sulfate  Deisenhofer et al., 1995 (1PRC)


Lancaster & Michel, 1997 (2PRC, 3PRC, 4PRC), Lancaster & Michel, 1999 (5PRC, 6PRC, 7PRC)
Lancaster et al., 2000 (1DXR)
from Rhodobacter sphaeroides N,N-dimethyldodecylamine-N-oxide/- heptane-1,2,3-triol/ polyethylene glycol 4000  Allen et al., 1987 (4RCR)
" octyl-ß-D-glucopyranoside/-/ polyethylene glycol 4000  Chang et al., 1991 (2RCR)



" N,N-dimethyldodecylamine-N-oxide/ heptane-1,2,3-triol, dioxane/ potassium phosphate  Ermler et al., 1994 (1PCR), Camara-Artigas et al., 2002 (1M3X)



" octyl-ß-D-glucopyranoside/ benzamidine, heptane-1,2,3-triol/ polyethylene glycol 4000 Stowell et al., 1997 (1AIG, 1AIJ)



" N,N-dimethyldodecylamine-N-oxide/ heptane-1,2,3-triol/ 
sodium citrate		 McAuley et al., 1999 (1QOV)
" (type I crystal grown in cubic lipidic phase of monoolein in the presence of ammonium sulphate Katona et al., 2003 (1OGV)
from Rhodobacter sphaeroides complexed with cytochrome c2 N,N-dimethyldodecylamine-N-oxide/ heptane-1,2,3-triol/ polyethylene glycol 4000 Axelrod et al., 2002 (1L9J, 1L9B)
from Thermochromatium tepidum octyl-ß-D-glucopyranoside/ - / polyethylene glycol 4000, NaCl Nogi et al., 2000 (1EYS)
Photosystem II from Thermosynechococcus vulcanus dodecyl-ß-D-maltoside/ - / polyethylene glycol 1450 Kamiya & Shen, 2003 (1IZL)
from Thermosynechococcus elongatus (?) dodecyloctaoxyethylene plus dodecyl-ß-D-maltoside / glycerol / polyethylene glycol 4000 Ferreira et al., 2004 (1S5L)
Photosystem I from Synechococcus elongatus dodecyl-ß-D-maltoside/ - / water (reverse of salting in) Jordan et al., 2001 (1JB0)
Bacterial rhodopsins
Bacteriorhodopsin from Halobacterium salinarum (electron crystallography using naturally occuring two-dimensional crystals)  Henderson et al., 1990 (1BRD) Grigorieff et al., 1996 (2BRD) Kimura et al., 1997 (1AT9)
" (type I crystal grown in cubic lipidic phase of monooleyl- rac-glycerol in the presence of sodium/potassium phosphate)  Pebay-Peyroula et al., 1997 (1AP9) Luecke et al., 1998 (1BRX), Luecke et al., 1999 (1C3W) Belrhali et al., 1999 (1QHJ)
" octyl-ß-D-glucopyranoside/benzamidine/ sodium phosphate (epitaxial growth on benzamidine crystals)  Essen et al., 1998 (1BRR)
" (type I crystal obtained by bicelle crystallization) Faham & Bowie, 2002 (1KME)



Halorhodopsin 
from Halobacterium salinarum		 (type I crystal grown in cubic lipidic phase of monooleyl- rac-glycerol in the presence of KCl) Kolbe et al., 2000 (1E12)
Sensory Rhodopsin II 
from Natronobacterium pharaonis		 (type I crystal grown in cubic lipidic phase of monoolein glyceride in the presence of KCl) Luecke et al., 2001 (1JGJ)
" (type I crystal grown in cubic lipidic phase of monoolein glyceride in the presence of  NaCl) Royant et.al., 2001 (1H68)
complexed with transducer fragment (type I crystal grown in cubic lipidic phase of monovaccenin in the presence of Na,K-phosphate) Gordeliy et al., 2002 (1H2S)
Sensory Rhodopsin form Anabaena
 (type I crystal grown in cubic lipidic phase of monolein glyceride in the presence of sodium citrate, magnesium chloride and MPD)
 Vogeley et al. 2004 (1XIO)



Light-harvesting complex II
from pea chloroplasts (electron crystallography of two-dimensional crystals prepared from Triton X100 solubilized material)  Kuehlbrandt et al., 1994 
from spinach chloroplasts nonyl-ß-D-glucopyranoside / digalactosyl diacyl glycerol plus N,N-bis- (3-D-gluconamido propyl) deoxycholamide / sodium citrate Liu et al., 2004 (1RWT)



Bacterial light-harvesting complexes
Light-harvesting complex 2 
from Rhodopseudomonas acidophila		 octyl-ß-D-glucopyranoside/benzamidine/ phosphate  McDermott et al., 1995 (1KZU), Papiz et al., 2003 (1NKZ)
from Rhodospirillum molischianum N,N-dimethylundecylamine-N-oxide/ heptane-1,2,3-triol/ ammonium sulfate  Koepke et al., 1996 (1LGH)
Light-harvesting complex 3 from Rhodopseudomonas acidophila octyl-ß-D-glucopyranoside/ benzamidine/ phosphate McLuskey et al., 2001 (1IJD)



Cytochrome c oxidase / respiratory complex IV / ubiquinol oxidase:
Cytochrome c oxidase 
from Paracoccus denitrificans:

four-subunit enzyme complexed with antibody Fv-fragment,  dodecyl-ß-D-maltoside/-/ polyethylene glycol- monomethylether 2000  Iwata et al., 1995; Harrenga & Michel, 1999 (1QLE)
two-subunit enzyme complexed with antibody Fv-fragment. undecyl-ß-D-maltoside/-/ polyethylene glycol- monomethylether 2000  Ostermeier et al., 1997 (1AR1)
from bovine heart mitochondria decyl-ß-D-maltoside with some residual cholate/-/ polyethylene glycol 4000  Tsukihara et al., 1996 (1OCC) Yoshikawa et al., 1998 (2OCC, 1OCR), Tsukihara et al., 2003 (1V54, 1V55)
from Thermus thermophilus nonyl-ß-D-glucoside/-/ polyethylene glycol 2000 Soulimane et al., 2000 (1EHK)
from Rhodobacter sphaeroides decyl-ß-D-maltoside, dodecyl-ß-D-maltoside/ heptane-1,2,3-triol/ polyethylene glycol 400 Svensson-Ek et al., 2002 (1M56, 1M57)
ubiquinol oxidase (cytochrome bo) from Escherichia coli octyl-ß-D-glucoside/ethanol/ polyethylene glycol 1500, MgCl2 Abramson et al., 2000 (1FFT)



Cytochrome bc1 complex / respiratory complex III / cytochrome b6f complex
Cytochrome bc1 complex from bovine heart mitochondria  decanoyl-N-methyl-glucamide or diheptanoyl phosphatidyl choline /-/ polyethylene glycol 4000  Xia et al., 1997 (1QCR), Kim et al., 1998 

octyl-ß-D-glucopyranoside /-/ polyethylene gycol 4000  Zhang et al., 1998

pure dodecyl-ß-D-maltoside or mixture with methyl-6-O-(N-heptylcarbamoyl) -alpha-D- glucopyranoside/ polyethylene glycol 4000 Iwata et al., 1998 (1BE3, 1BGY)
from chicken heart mitochondria  octyl-ß-D-glucopyranoside /- / polyethylene glycol 4000 Zhang et al., 1998 (1BCC, 3BCC)
from the yeast Saccharomyces cerevisiae complexed with antibody Fv-fragment undecyl-ß-D-maltoside /-/ polyethylene glycol 4000 Hunte et al., 2000 (1EZV),
Lange et al. 2001 (1KB9)
complexed with cytochrome c and antibody Fv-fragment " Lange & Hunte, 2002 (1KYO)
from Rhodobacter capsulatus
 undecyl-ß-D-maltoside/ heptanetriol/ polyethylene glycol 400
 Berry et al., 2004
Cytochrome b6f complex 
from Mastigocladus laminosus		 undecyl-ß-maltoside/dioleylphosphadidyl - choline / polyethylene glycol 400 Kurisu et al., 2003 (1UM3)
from Chlamydomonas reinhardtii dodecyl-ß-D-maltoside / glycerol / polyethylene - glycol monomethylether 350 Stroebel et al., 2003 (1Q90)



Fumarate reductase/succinate dehydrogenase / respiratory complex II
Fumarate reductase from Escherichia coli dodecyl nonaoxyethylene/-/ polyethylene glycol 1000 Iverson et al., 1999 (1FUM)

dodecyl nonaoxyethylene/-/ polyethylene glycol 5000 
dodecyl nonaoxyethylene/-/ polyethylene monomethylether		 Iverson et al., 2002 (1KF6, 1KFY, 1L0V)
Fumarate reductase from Wolinella succinogenes mixture of dodecyl-ß-D-maltoside and decyl-ß-D-maltoside/benzamidine/ polyethylene glycol 3350 Lancaster et al., 1999 (1QLA,1QLB), Lancaster et al., 2001 (1E7P)
Succinate dehydrogenase from Escherichia coli dodecyl nonaoxyethylene/ ethylene glycol/ polyethylene glycol 400 Yankovskaya et al., 2003 (1NEK, 1NEN) 



Potassium channels
KcsA from Streptomyces lividans  N,N-dimethyldodecylamine-N-oxide/-/ polyethylene glycol 400  Doyle et al., 1998 (1BL8)
KcsA from Streptomyces lividans complexed with Fab antibody fragment  decyl-ß-D-maltoside/-/ polyethylene glycol 400 Zhou et al., 2001 (1K4L, 1K40), Valiyaveetil et al., 2002 (1K4C)
Calcium gated potassium channel from Methanobacterium thermoautotrophicum N,N-dimethyldodecylamine-N-oxide/-/ polyethylene glycol 350 monomethylether Jiang et al., 2002 (1LNQ)
Voltage-dependent K+ channel from Aeropyrum pernix octyl-ß-D-glucopyranoside/-/ polyethylene glycol 400 or polyethylene glycol 350 mono methylether Jiang et al., 2003 (1ORS, 1ORQ)
KirBac 1.1 from Burkholderia pseudomallei tridecyl-ß-D-maltoside, Cymal-4-mixture /-/ polyethylene glycol 400 Kuo et al., 2003 (1P7B)



Large Mechanosensitive ion channel MscL
from Mycobacterium tuberculosis dodecyl-ß-D-maltoside/-/ triethylene glycol  Chang et al., 1998 (1MSL)



Small Mechanosensitive ion channel MscS
from Escherichia coli Foscholine-14 /-/ polyethylene glycol 3350 Bass et al., 2002 (1MXM)



P-type ATPases
Ca-ATPase from rabbit sarcoplasmic reticulum (E1-state) (type I crystal obtained by removing the detergent dodecyl octaoxyethylene by dialysis) Toyoshima et al., 2000 (1EUL)
Ca-ATPase from rabbit sarcoplasmic reticulum (E2-state) " Toyoshima and Nomura, 2002 (1IWO)
Ca-ATPase from rabbit sarcoplasmic reticulum (nucleotide complexes) dodecyloctaoxyethylene / t-butanol / polyethylene glycol 6000 Sorensen et al., 2004 (1T5S, 1T5T)
Ca-ATPase from rabbit sarcoplasmic reticulum complexed with MgF 2-4 
(type I crystal obtained by removing the detergent dodecyl octaoxyethylene by dialysis) Toyoshima et al.,  2004 (1WPE, 1WPG)



G-protein coupled receptors
Rhodopsin from bovine rod outer segments nonyl-ß-D-glucopyranoside/ heptane-1,2,3-triol/ ammonium sulfate Palczewski et al., 2000 (1F88), Teller et al., 2001 (1HZX), Okada et al., 2002 (1L9H)
"
 heptylthioglucoside/ - /ammonium sulfate
 Okada et al., 2004 (1U19)
" octyltetraoxyethylene / N,N - dimethyldodecylamine- N - oxide / lithium sulfate, polyethylene glycol 8000
 Li et al., 2004 (1GZM)
Aquaporins and relatives
Aquaporin AQP1 from human red blood cells  (electron crystallography of two-dimensional crystals)  Murata et al., 2000 (1FQY), Ren et al,, 2001 (1HW0)
Aquaporin AQP1 from bovine red blood cells nonyl-ß-D-glucopyranoside/-/ polyethylene glycol monomethylether 550 Sui et al., 2001 (1J4N)
Glycerol facilitator GlpF from Escherichia coli octyl-ß-D-glucopyranoside/dithiothreitol/ polyethylene glycol 2000, MgCl2 Fu et al., 2000 (1FX8)
Aquaporin Z from Escherichia coli octyl-ß-D-glucopyranoside/ isopropanol/ polyethylene glycol monomethylether 2000 Savage et al., 2003 (1RC2)
Aquaporin 0 from bovine eye lenses
 nonyl-ß-D-glucopyranoside/ - /polyethylene glycol 1000
 Harries et al., 2004 (1TM8)



Chloride channels
from Salmonella typhimurium octyl-ß-D-maltoside/ - /polyethylene glycol 400 Dutzler et al., 2002 (1KPK)
from Escherichia coli " Dutzler et al., 2002 (1KPL)
"   complexed with antibody fragment decyl-ß-D-maltoside/ - /polyethylene glycol 300 Dutzler et al., 2003 (1OTS, 1OTT, 1OTU)
Formate dehydrogenase-N 
from Escherichia coli octyl-ß-D-glucopyranoside/ethanol/ polyethylene glycol 1500 Jormakka et al., 2002 (1KQF, 1KQG)
ABC transporters
BtuCD from Escherichia coli N,N-dimethyldodecylamine-N-oxide/ 2-methyl-2,4-pentanediol/ 
polyethylene glycol 2000		 Locher et al., 2002 (1L7V)
Multidrug transporter 
MsbA from Vibrio cholerae		 dodecyl-ß-D-maltoside or tridecyl-ß-D-maltoside/ - /polyethylene glycol 400 Chang, 2003 (1PF4)
H+-coupled efflux transporter
AcrB from Escherichia coli dodecyl-ß-D-maltoside/ - /polyethylene glycol 2000 Murakami et al., 2002 (1IWG)
"  complexed with ligands
Yu et al., 2003 (1OY6, 1OY8, 1OY9, 1OYD, 1OYE)
Major facilitator superfamily transporters
Glycerol-3-phosphate transporter GlpT from Escherichia coli dodecyl-ß-D-maltoside plus dodecyloctaoxyethylene / - / polyethylene glycol 2000 monomethylether Huang et al., 2003 (1PW4)
Lactose permease LacY from Escherichia coli dodecyl-ß-D-maltoside plus CHAPS / 1,6-hexanediol / polyethylene glycol 400 Abramson et al., 2003 (1PV6, 1PV7)



Nicotinic acetylcholine receptor

from Torpedo marmorata (electron microscopy of tubular crystals) Miyazawa et al., 2003 (1OED)



Nitrate reductase A (Nar GHI)

from Escherichia coli dodecylnonaoxyethylene / - / polyethylene glycol 3000 Bertero et al., 2003 (1Q16)
ADP/ATP carrier

from bovine heart mitochondria 3-laurylamido-N,N' - dimethylpropylaminoxide / - / Jeffamine-M600 Pebay-Peyroula et al., 2003 (1OKC)



Small multidrug resistance transporters EmrE 
from Escherichia coli nonyl-ß-D-glucopyranoside/ - polyethylene glycol 200 Ma & Chang, 2004 (1S7B)



Protein-conducting channels
SecY complex from Methanococcus jannaschii 1,2-diheptanoyl-sn-glycero-3-phospho- choline/ - polyethylene glycol 400 van den Berg et al., 2004 (1RHZ, 1RH5)



Ammonium transporter family
Ammonium transport protein AmtB
from Escherichia coli
 octyl-ß-D-glucopyranoside / - / polyethylene glycol
 Khademi et al., 2004 (1U77, 1U7G, 1U7C)
"
 N,N-dimethyldodecylamine-N-oxide / Deoxy- BigCHAP / polyethylene glycol 550
 Zheng et al., 2004 (1XQE, 1XQF)



Dicarboxylate Amino Acid Symporter (DAACS) family
Glutamate transporter homologue from  Pyrococcus horikoshii
 decyl-ß-D-maltoside / - / polyethylene glycol 1000
 Yernool et al., 2004 (1XFH)



Methane Monooxygenases
Methane Monoxygenase from Methylococcus capsulatus
 cyclohexylpentyl-ß-D-maltoside (Cymal-5) / - / polyethylene glycol 8000
 Lieberman & Rosenzweig, 2005 (1YEW)






B. Membrane proteins from the outer membrane of gram negative bacteria and related membrane proteins



16-stranded porins
from Rhodobacter capsulatus octyltetraoxyethylene/-/polyethylene glycol 600  Weiss et al., 1991 (2POR)
ompF and PhoE from Escherichia coli mixture of n-octyl-2-hydroxyethylsulfoxide and octylpolyoxyethylene; 
or N,N-dimethyldecylamine-N-oxide/-/ polyethylene 
glycol 2000 		 Cowan et al., 1992 (1OPF, 1PHO)Cowan et al., 1995 
from Rhodopseudomonas blastica octyltetraoxyethylene/ heptane-1,2,3-triol/ polyethylene 
glycol 600 		 Kreusch et al., 1994 (1PRN)
from Paracoccus denitrificans octyl-ß-D-glucopyranoside/-/ polyethylene glycol 600  Hirsch et al., 1997 
OmpK36 (osmoporin) from Klebsiella pneumoniae

anion selective Omp32 from Comamomonas acidovorans

 2-hydroxyethyloctylsulfoxide/-/ polyethylene glycol 2000 
 

octyl-ß-D-glucopyranoside/-/ Li2SO4,(NH4)2SO4

 Dutzler et al., 1999 (1OSM)

Zeth et al., 2000 (1E54)
18-stranded porins
Maltoporin from Escherichia coli mixture of decyl-ß-D-maltoside and dodecyl nonaoxyethylene/-/ 
polyethylene glycol 2000		 Schirmer et al., 1995 (1MAL)
Maltoporin from Salmonella typhimurium mixture of octyltetraoxyethylene and N,N- dimethylhexylamine-N-oxide /-/ polyethylene glycol 1500  Meyer et al., 1997 (1MPR, 2MPR)
Sucrose specific ScrY porin from Salmonella typhimurium mixture of octyl-ß-D-glucopyranoside and N,N- dimethylhexylamine-N-oxide /-/ polyethylene glycol 2000  Forst et al., 1998 (1A0S,1A0T)
alpha-hemolysin

from Staphylococcus aureus octyl-ß-D-glucopyranoside/ - / ammonium sulfate, polyethylene glycol monomethylether 5000 Song et al., 1996 (7AHL)



8-stranded ß-barrel membrane proteins
OmpA-fragment from Escherichia coli octyltetraoxyethylene/2-propanol, hexyldimethylamine-N-oxide/ 
ammonium sulfate		 Pautsch & Schulz, 1998, 2000 (1BXW, 1QJP)
OmpX from Escherichia coli octyltetraoxyethylene/-/2-propanol Vogt & Schulz, 1999 (1QJ8, 1QJ9)
NspA from Neisseria meningitidis decylpentaoxyethylene / isopropanol / polyethylene glycol 3000 Vandeputte et al., 2003 (1P4T)
acyltransferase Pag P from Escherichia coli
 N,N-dimethyldodecylamine-N-oxide /-/ 2-methyl-2,4-pentanediol
 Ahn et al., 2004 (1THQ)



22-stranded receptors
FhuA from Escherichia coli N,N-dimethyldecylamine-N-oxide /inositol/ polyethylene glycol monomethylether 2000 Ferguson et al., 1998 (1FCP, 2FCP)

n-octyl-2-hydroxyethylsulfoxide /-/ polyethylene glycol 2000  Locher et al., 1998 (1BY3, 1BY5)
complexed with lipopolysaccharide 

complexed with
rifamycin derivative

 N,N-dimethyldecylamine-N-oxide /inositol/ polyethylene glycol monomethylether 2000 

                                      "

 Ferguson et al., 2000 (1QFF, 1QFG)

Ferguson et al., 2001 (1FI1)
Ferric enterobacterin receptor (fepA) from Escherichia coli N,N-dimethyldodecylamine-N-oxide/ heptane-1,2,3-triol/ 
polyethylene glycol 1000		 Buchanan et al., 1999 (1FEP)
ferric citrate transporter (FecA)
from Escherichia coli		 N,N-dimethyldodecylamine-N-oxide/ heptane-1,2,3-triol/ 
polyethylene glycol 1000		 Ferguson et. al., 2002 (1KMO, 1KMP)
cobalamine transporter BtuB 
from Escherichia coli		 octyltetraoxyethylene / - / polyethylene glycol 3350 Chimento et al., 2003 (1NQF, 1NQE, 1NQG, 1NQH)
pyoverdine receptor FpvA from Pseudonomas aeruginosa
 octylpentaoxyethylene / - / polyethylene glycol 4000
 Cobessi et al., 2005 (1XKH)



12-stranded ß-barrel outer membrane proteins
outer membrane anchored phospholipase A (OMPLA) from Escherichia coli octyl-ß-D-glucopyranoside / - / 2-methyl-2,4-pentanediol Snijder et al., 1999 (1QD5, 1QD6)
nucleoside transporter Tsx
from Escherichia coli
 octyltetraoxyethylene / - / polyethylene glycol monomethylether 550
 Ye et al., 2004 (1TLY, 1TLW, 1TLZ)
translocator domain of autotransporter NalP from Neisseria meningitidis
 decylpentaoxyethylene/ heptyl-ß-D-glucopyranoside/ polyethylene glycol 1000
or polyethylene glycol monomethylester 2000 plus 2-methyl-2,4-pentanediol
 Oomen et al., 2004 (1UYN, 1UYO)



TolC channel (3x 4ß-strands) and related proteins
from Escherichia coli: n-dodecyl-ß- D-glucopyranoside, n-hexyl-ß-D-glucopyranoside, n-heptyl-ß-D-glucopyranoside, n-octyl-ß-D-glucopyranoside/ heptane-1,2,3-triol/ polyethylene glycol monomethylether 2000 Koronakis et al., 2000 (1EK9)
 "   complexed with Co(NH3)3+6
" Higgins et al., 2004 (1TQQ)



OprM
 from Pseudomonas aeruginosa
 cyclohexylpropyl-ß-D-maltoside, octylpolyoxyethylene / - / 2-methyl-2,4-pentanediol
 Akama et al., 2004 (1WP1)



10-stranded ß-barrel outer membrane proteins
protease OmpT 
from Escherichia coli		 octyl-ß-D-glucopyranoside/ - /2- methyl- 2,4-pentanediol Vandeputte-Rutten et al., 2001 (1I78)
Outer-membrane adhesin OpcA 
from Neisseria meningitidis		 decylpentaoxyethylene/ heptyl-ß-D-glucoside/ polyethylene glycol 4000 Prince et al., 2002 (1K24)



2-stranded ß-barrel outer membrane proteins forming an octameric channel
MspA from 
Mycobacterium smegmatis		 octyltetraoxyethylene plus decanoyl- N- hydroxy ethylglucamide/ - /polyethyleneglycol 4000 Faller et al., 2004 (1UUN)



14-stranded ß-barrel outer membrane protein
long-chain fatty acid transporter FadL from Escherichia coli octyltetraoxyethylene plus traces of N,N- dimethyldodecylamine-N-oxide/ - /polyethylene glycol 4000 or magnesium formate van den Berg et al., 2004 (1T16, 1T1L)






C. Proteins inserted into, but not crossing the membrane ("monotopic membrane proteins")



Prostaglandin H2 synthase-1 (cyclooxygenase 1)
from sheep  octyl-ß-D-glucopyranoside/-/ polyethylene glycol 4000  Picot et al., 1994 (1PRH), Malkowski et al., 2000 (1DIY), Malkowski et al., 2001 (1IGX, 1IGZ)
Cyclooxygenase-2 

from mouse octyl-ß-D-glucopyranoside/-/ polyethylene glycol monomethylether 550 Kurumbail et al., 1996 (1CX2, 3PGH, 4COX, 5COX, 6COX) 
Kiefer et al., 2000 (1DDX)
from man octylpentaoxyethylene/-/ polyethylene glycol 4000  Luong et al., 1996 
Squalene cyclase 

from Alicyclobacillus acidocaldarius octyltetraoxyethylene/-/ sodium citrate Wendt et al., 1997 (1SQC)



Fatty acid amide hydrolase from rat N,N-dimethyldodecylamine-N-oxide/ methylpentanediol/ polyethylene glycol 6000 Bracey et al., 2002 (1MT5)






D. Membrane proteins of uncertain classification
Monoamine oxidase B
from man		 (1) N,N-dimethyldodecylamine-N-oxide/-/ polyethylene glycol 4000 Binda et al., 2002 (1GOS), 2003 (1OJA, 1OJB, 1OJC, 1OJD, 1OJ9)
It is unclear whether the C-terminal membrane anchoring segments does span (A) the membrane or not (C).




 
 


STATISTICS OF CRYSTALLIZATION CONDITIONS:
 
Detergents used: total: A: B: C: D:
N,N-dimethyldodecylamine-N-oxide 14 9
 3
 1 1
N,N-dimethylundecylamine-N-oxide 1 1 - -
N,N-dimethyldecylamine-N-oxide 3 - 3 -
N,N-dimethylhexylamine-N-oxide (as additive) 3 - 3 -






n-dodecyl-N,N-dimethyl-3-amino-1-propanesulfonate (zwittergent 3-12) 1 - - - 1
nonyl-ß-D-glucopyranoside 6
 6
 - -
octyl-ß-D-glucopyranoside 21 13 6 2
heptylthioglucopyranoside
 1
 1
 -
 -
glucopyranoside mixture 1 1 - -






decanoyl-N-methyl glucamide 1 1 - -






tridecyl-ß-D-maltoside/Cymal-4 mixture 1 1 - -
tridecyl-ß-D-maltoside 1 1 - -
dodecyl-ß-D-maltoside 11 11 - -
undecyl-ß-D-maltoside 5
 5
 - -
decyl-ß-D-maltoside 5 4 1 -
octyl-ß-D-maltoside 2 2 - -
octyl-2-hydroxy ethylsulfoxide 3 - 3 -
cyclohexylpropyl-ß-D-maltoside (Cymal-3)
 1
 -
 1
 -
cyclohexylpentyl-ß-D-maltoside (Cymal-5)
 1
 1
 -
 -






Foscholine-14 1 1 - -
CHAPS (as additive) 1 1 - -






methyl-6-O-(N-heptylcarbamoyl)-alpha-D-glucopyranoside (as additive) 1 1 - -






dodecyl nonaoxyethylene ("thesit") 4 3 1 -
dodecyl octaoxyethylene (as additive) 1 1 - -
dodecyl octaoxyethylene 2 2 - -
decylpentaoxyethylene 3 - 3 -
octyltetraoxyethylene 9
 1
 7
 1
octyltetraoxyethylene/ decanoyl-N-hydroxyethylglucamide mixture 1 - 1 -
octylpentaoxyethylene 2 - 1
 1
octylpolyoxyethylene 2
 - 2
 -






1,2-diheptanoyl-sn-glycero-3-phosphocholine 1 1 - -






3-laurylamido-N,N'-dimethylpropylamine oxide ("LAPAO") 1 1 - -






cubic lipidic phase of monooleyl-rac-glycerol 5
 5
 - -
cubic lipidic phase of monovaccenin 1 1 - -






bicelle crystallization 1 1 - -






detergent removal by dialysis 1 1 - -












Additives:




heptane-1,2,3-triol 13 9
 4 -
benzamidine 4 4 - -
heptyl-ß-D-glucoside 2 - 2 -
dioxane 1 1 - -
ethanol 1 1 - -
ethylene glycol 1 1 - -
2-propanol 1 - 1 -
isopropanol 2 1 1 -
t-butanol 1 1 - -
inositol 1 - 1 -
glycerol 1 1 - -
2-methyl-2,4-pentanediol 1 1 - 1
1,6-hexanediol 1 1 - -
dioleylphosphatidylcholine 1 1 - -
digalactosyl diacylgycerol 1 1 - -
N,N-bis-(3-D-glyconamidopropyl) deoxycholamide 1 1 - -
Deoxy-Big CHAP
 1
 1
 -
 -












Precipitants:




ammonium sulfate 5 3 2 1
lithium sulfate 2
 1 1 -
phosphates 4 4 - -
citrates 3 2 - 1
formates 1 - 1 -






polyethylene glycol 8000
 1
 1
 -
 -
polyethylene glycol 6000 2 1 - 1
polyethylene glycol 4000 20 13 4
 2 1
polyethylene glycol 3350 3 2 1 -
polyethylene glycol 3000 2 1 1 -
polyethylene glycol 2000 8 3 5 -
polyethylene glycol 1500 3 2 1 -
polyethylene glycol 1450 1 1 - -
polyethylene glycol 1000 5
 2
 3
 -
polyethylene glycol 600 3 - 3 -
polyethylene glycol 550
 1
 1
 -
 -
polyethylene glycol 400 11 11 - -
polyethylene glycol 200 1 1 - -
triethylene glycol 1 1 - -
polyethylene glycol monomethylether 5000 2 1 1 -
polyethylene glycol monomethylether 2000 9
 5 4
 -
polyethylene glycol monomethylether 550 3
 1 1
 1
polyethylene glycol monomethylether 350 3 3 - -






Jeffamine-M600 (O-(2-aminopropyl)-O'-(2 methoxyethyl) polypropylene glycol 500) 1 1 - -






2-propanol 1 - 1 -
2-methyl-2,4-pentanediol 5
 - 5
 -







 
 
 

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